Anti-Golgin 97 GOLGA1 Monoclonal Antibody

• Catalog Number: orb548495
• Category: Antibodies
• Description: Anti-Golgin 97 GOLGA1 Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: ACAD-7
• Tested applications: IP, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Golgin 97 The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome. The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker.
• Antibody Type: Primary Antibody
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 18 kDa
• UniProt ID: Q92805
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Histone H3.1; Histone H3/a; Histone H3/b; Histone
• Note: For research use only
• Application notes: WB 1:1000-1:5000IP 1:30. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GOLGA1 expression in HeLa cell lysate using anti-GOLGA1 antibody (orb548495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLGA1 antigen affinity purified polyclonal antibody (Catalog # orb548495) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for GOLGA1.