Anti-GNGT1 Antibody

• Catalog Number: orb1289731
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to GNGT1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Mouse, Rat
• Immunogen: Recombinant protein corresponding to human GNGT1. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:1000, IF:IC: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GNGT1
• Entrez: 14699, 680149
• UniProt ID: Q61012, D4AAI1
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Guanine nucleotide-binding protein G(T) subunit ga
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GNGT1 expression in mouse eyes (A), rat eyes (B) whole cell lysates. (Predicted band size: 8 kD; Observed band size: 10 kD)

Immunofluorescent analysis of GNGT1 staining in mouse eyes. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).