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Anti-GLUT4 Antibody

Catalog Number: orb1474177

DispatchUsually dispatched within 5-10 working days
$ 240.00
Catalog Numberorb1474177
CategoryAntibodies
DescriptionMouse monoclonal antibody to GLUT4
Species/HostMouse
ClonalityMonoclonal
Tested applicationsFC, IF, IH, WB
ReactivityHuman, Mouse
ImmunogenRecombinant fragment of human GLUT4 expressed in E. Coli. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/100 - 1/500), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetSLC2A4
Entrez6517, 20528
UniProt IDP14142, P14672
SourceMouse
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesGLUT4; Solute carrier family 2, facilitated glucos
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-GLUT4 Antibody

Western blot analysis of GLUT4 expression in HeLa (A), NIH3T3 (B), 3T3-L1 (C), mouse heart (D) whole cell lysates. (Predicted band size: 43; 54 kD; Observed band size: 55 kD)

Anti-GLUT4 Antibody

Immunohistochemical analysis of GLUT4 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-GLUT4 Antibody

Immunofluorescent analysis of GLUT4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Anti-GLUT4 Antibody

Flow cytometric analysis of HeLa cells using Anti-GLUT4 Antibody (green) and negative control (purple).

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