GLUT4 Antibody

• Catalog Number: orb1474177
• Category: Antibodies
• Description: The GLUT4 Antibody is suitable for FC, IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fragment of human GLUT4 expressed in E. Coli. The exact sequence is proprietary. Purification: The antibody was purified by protein G.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by protein G.
• Immunogen: Recombinant fragment of human GLUT4 expressed in E. Coli. The exact sequence is proprietary.
• UniProt ID: P14672, P14142
• Tested applications: FC, IF, IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/100 - 1/500), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: GLUT4; Solute carrier family 2, facilitated glucos
• Note: For research use only
• Entrez: 6517, 20528

Western blot analysis of GLUT4 expression in HeLa (A), NIH3T3 (B), 3T3-L1 (C), mouse heart (D) whole cell lysates. (Predicted band size: 43; 54 kD; Observed band size: 55 kD)

Immunohistochemical analysis of GLUT4 staining in human bladder cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GLUT4 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of HeLa cells using Anti-GLUT4 Antibody (green) and negative control (purple).