GluR2 Monoclonal Antibody

SKU: orb548127

Description

Anti-GluR2 Monoclonal Antibody. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Neuroscience, Protein Biochemistry

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution range: WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:20
• Reactivity: Human, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: ACIB-7
• Immunogen: A synthesized peptide derived from human GluR2 Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
• Target: Glutamate receptor 2
• Molecular Weight: 136 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin.
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

RNA-binding protein Musashi homolog 2; Musashi-2; MSI2

Western blot analysis of GRIA2 expression in (1) Human fetal brain lysate; (2) Mouse brain lysate using anti-GRIA2 antibody (orb548127). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA2 antigen affinity purified polyclonal antibody (Catalog # orb548127) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for GRIA2.

Quick Database Links

Gene Symbol

Glutamate receptor 2

UniProt

P42262