Anti-GDF8 Antibody

• Catalog Number: orb378184
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MSTN.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human GDF8
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MSTN
• Entrez: 17700, 2660, 29152
• UniProt ID: O14793, O08689, O35312
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: GDF8; Growth/differentiation factor 8; GDF-8; Myos
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GDF8 expression in U937 (A), BT474 (B), mouse heart (C), mouse liver (D), rat skeletal muscle (E) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)

Immunohistochemical analysis of GDF8 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.