Anti-GCN4 [C11L34]

SKU: orb613848

Description

Mouse monoclonal antibody to GCN4

Research Area

Epigenetics

Images & Validation

• Tested Applications: IP, WB
• Reactivity: Yeast

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: C11L34
• Immunogen: Biotinylated GCN4-peptide (HLENEVARLKK).
• Target: GCN4
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

General Control Protein 4

ELISA using C11L34 and Multi Tag protein 2. Binding curves of the mouse IgG1 chimeric version of the Anti-GCN4 antibody C11L34 (orb613848; grey line) and isotype control (orb256381, anti-fluorescein; orange line) to an ELISA plate coated with Multi Tag protein 2 at a concentration of 5 ug/ml.

Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34. Immunofluorescence analysis of fixed HeLa cells stained with the chimeric mouse IgG1 version of C11L34 (orb613848) followed by an Alexa Fluor® 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is DAPI (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) orb613848 with Alexa Fluor® 488 secondary antibody, c) DNA stained with DAPI, d) Merged channels.

Immunofluorescence staining of fixed HeLa cells expressing mCherry tagged GCN4 with anti-GCN4 antibody C11L34. Immunofluorescence analysis of fixed HeLa cells stained with the chimeric rabbit IgG version of C11L34 (orb613849) followed by an Alexa Fluor® 488 secondary antibody, showing mitochondrial staining. Colocalisation with mCherry tagged GCN4 demonstrates the very low off target/non-specific binding of this antibody. The nuclear and mitochondiral DNA stain is hoechst (blue). Panels show; a) mCherry tagged, mitochondrially targetted GCN4, b) orb613849 with Alexa Fluor® 488 secondary antibody, c) DNA stained with hoechst, d) Merged channels.

Quick Database Links

Gene Symbol

GCN4

UniProt

P03069