GAPDHS Antibody

• Catalog Number: orb43877
• Category: Antibodies
• Description: Mouse Monoclonal to Intra-Acrosomal Protein.
• Target: GAPDHS
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Conjugation: Unconjugated
• Reactivity: Human, Porcine
• Concentration: 1 mg/ml
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• UniProt ID: O14556
• RRID: AB_10995916
• Tested applications: FC, ICC, WB
• Application notes: Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential. Flow cytometry: Recommended dilution: 3-12 µg/ml. Intraacrosomal staining.
• Antibody Type: Primary Antibody
• Clone Number: Hs-8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SGAPDH, HSD-35
• Note: For research use only
• Entrez: 26330

Separation of human GAPDHS positive cells (red-filled) from GAPDHS negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 μg/ml) GAM APC.

Flow cytometry intracellular staining pattern of human sperm cells stained using anti-GAPDHS (Hs-8) purified antibody (concentration in sample 7.5 μg/ml) GAM APC.