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Anti-GAP43 (Phospho-S41) Antibody
Catalog Number: orb382520
| Catalog Number | orb382520 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to GAP43 |
| Target | GAP43 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Primate, Rat, Zebrafish |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Purity | The antibody was purified by immunogen affinity chromatography |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S41 of human GAP43 protein. The exact sequence is proprietary. |
| UniProt ID | P17677, P07936, P06837 |
| Tested applications | IF, WB |
| Dilution range | WB: 1:500:2000, IF/ICC: 1:50:100 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti-Neuromodulin antibody, anti-Axonal membrane p Read more... |
| Note | For research use only |
| Entrez | 29423, 2596, 14432 |
Western blot analysis of GAP43 (Phospho-S41) expression in HeLa PMA-treated (A), SHSY5Y (B) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)
Immunofluorescent analysis of GAP43 (Phospho-S41) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
