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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, ICC, IHC, WB |
|---|---|
| Dilution Range | WB: 1.430555556, IHC: 1:500-1:1000, ICC: 1:500-1:2000 |
| Reactivity | Bovine, Equine, Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Gallus |
| Clonality | Polyclonal |
| Isotype | IgY |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GAP43, conjugated to keyhole limpet hemocyanin (KLH). |
| Target | GAP43 |
| Molecular Weight | 43 |
| Purification | Total IgY fraction |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS + 10 mM NaN3. |
| Concentration | 15 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunofluorescence of rat neuron/glial culture labeling GAP43 protein (Cat. orb319547, 1:2000, green) expressed in the axonal membrane and synapses of neuronal cells. DAPI (blue) was used for nuclear staining.

Immunolabeling of mixed neuron and glial cultures with anti-GAP43 (Cat. orb319547, 1:2000, green), alpha II spectrin (Cat. orb319455, 1:1000, red) and nuclear staining with DAPI (blue). The anti-GAP43 labels protein expressed in the axonal membrane and synapses of neuronal cells. The Anti-αII-Spectrin labels the submembraneous cytoskeleton of axons and dendrites.

Western blot of rat cortical lysate showing specific immunolabeling of the ~43 kDa GAP43 protein.
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GAP43 Antibody (orb319547)
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