gamma Sarcoglycan Rabbit Monoclonal Antibody

• Catalog Number: orb548395
• Category: Antibodies
• Description: Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody. Tested in WB, IHC, IP applications. This antibody reacts with Human.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human gamma Sarcoglycan
• UniProt ID: Q13326
• MW: 74,76 kDa
• Tested applications: IHC, IP, WB
• Application notes: WB 1:500-1:2000IHC 1:50-1:200IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: AbS34
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Gamma-sarcoglycan; Gamma-SG; 35 kDa dystrophin-ass
• Note: For research use only

Western blot analysis of gamma Sarcoglycan expression in Human skeletal muscle lysate.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-gamma Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.