gamma Sarcoglycan Rabbit Monoclonal Antibody

SKU: orb548395

Description

Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB 1:500-1:2000 IHC 1:50-1:200 IP 1:50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AbS34
• Immunogen: A synthesized peptide derived from human gamma Sarcoglycan
• Target: Gamma-sarcoglycan
• Molecular Weight: 74,76 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Gamma-sarcoglycan; Gamma-SG; 35 kDa dystrophin-associated glycoprotein; 35DAG; SGCG

Western blot analysis of gamma Sarcoglycan expression in Human skeletal muscle lysate.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-gamma Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

Gamma-sarcoglycan

UniProt

Q13326