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Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody

Catalog Number: orb548395

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb548395
CategoryAntibodies
DescriptionAnti-gamma Sarcoglycan Rabbit Monoclonal Antibody. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityMonoclonal
Clone NumberAbS34
Tested applicationsIHC, IP, WB
ReactivityHuman
IsotypeRabbit IgG
ImmunogenA synthesized peptide derived from human gamma Sarcoglycan
Antibody TypePrimary Antibody
ConcentrationActual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Form/AppearanceLiquid
ConjugationUnconjugated
MW74,76 kDa
UniProt IDQ13326
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesGamma-sarcoglycan; Gamma-SG; 35 kDa dystrophin-ass
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NoteFor research use only
Application notesWB 1:500-1:2000IHC 1:50-1:200IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Expiration Date12 months from date of receipt.
Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody

Western blot analysis of gamma Sarcoglycan expression in Human skeletal muscle lysate.

Anti-gamma Sarcoglycan Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-gamma Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.