Anti-Gamma Delta TCR [B1.1]

• Catalog Number: orb2645762
• Category: Antibodies
• Description: Anti-Gamma Delta TCR [B1.1]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: B1.1
• Tested applications: Blocking, FA, FC, IF, IHC
• Reactivity: Human, Primate
• Isotype: IgG
• Immunogen: Antigen-specific T cell blasts to poly-18, a polypeptide antigen of defined sequence and conformation, were generated from lymph nodes of antigen primed BALB/cCr mice. These blasts were fused with the BW5147 thymoma to obtain anti-poly-18-reactive T cell hybridomas from which the original antibody was generated.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: Gamma Delta TCR
• UniProt ID: P04234, P09693
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TCR γδ; γδ TCR; TCR GD; GD TCR; TCRg/d; T-cell sur
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of Jurkat cells with anti-Gamma Delta TCR antibody B1.1. Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of B1.1 (orb2645762) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645762, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.