Anti-Galectin 9 [RG9-35]

• Catalog Number: orb669747
• Category: Antibodies
• Description: Mouse monoclonal antibody to Galectin 9
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: RG9-35
• Tested applications: Blocking, FA, FC, IP
• Reactivity: Mouse
• Isotype: Rat IgG
• Immunogen: This antibody was raised by immunising rats with recombinant mouse galectin-9.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Galectin 9
• UniProt ID: O08573
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ecalectin; Gal-9; galectin 9; Galectin-9; HUAT; le
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-Galectin 9 antibody RG9-35. NIH3T3 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of RG9-35 (orb669747, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed NIH3T3 cells with anti-Galectin 9 antibody RG9-35. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of RG9-35 (orb669747) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb669747, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.