GABP-alpha Rabbit Polyclonal Antibody

SKU: orb666734

Description

Rabbit polyclonal antibody to GABP-alpha

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-2000, IHC-P: 1-50-200, IF: 1-50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human GABP-alpha. The exact sequence is proprietary.
• Target: GABPA
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-E4TF1A antibody, anti-GA-binding protein alpha chain antibody, anti-GABP subunit alpha antibody, anti-Nuclear respiratory factor 2 subunit alpha antibody, anti-Transcription factor E4TF1-60 antibody

Western blot analysis of GABP-alpha expression in SW480 (A), mouse heart (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 56 kD)

Immunohistochemical analysis of GABP-alpha staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of GABP-alpha staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

GABPA

UniProt

Q06546, Q00422

NCBI Gene ID

14390, 2551