GABAA Receptor α2 Antibody

SKU: orb319526

Description

Our Anti-GABAA Receptor α2 rabbit polyclonal primary antibody from Biorbyt is produced in-house. It detects mouse GABAA Receptor α2 and is antigen affinity purified from pooled serum.

Research Area

Disease Biomarkers, Neuroscience

Images & Validation

• Tested Applications: ELISA, ICC, WB
• Dilution Range: WB: 0.736111111, ICC: 0.736111111
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Primate, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Fusion protein from the cytoplasmic loop of the α2 subunit of rat GABAA receptor.
• Target: GABAA Receptor α2
• Molecular Weight: 51
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GABA A receptor subunit alphα2 antibody, GABA antibody, GABA(A) receptor subunit alphα2 antibody, GABA(A) receptor subunit alpha-2 antibody, GABR A2 antibody, GABRα2 antibody, GABRA2 antibody, GABRA2 protein antibody, Gamma aminobutyric acid (GABA) A receptor alphα2 antibody, Gamma aminobutyric acid A receptor alphα2 antibody, Gamma aminobutyric acid receptor alphα2 subunit antibody, Gamma aminobutyric acid receptor subunit alphα2 antibody, Gamma-aminobutyric acid receptor subunit alpha-2 antibody, GBRA2_HUMAN antibody

Immunoblots showing GABAA Receptor α2 (Cat. orb319526) and GABAA Receptor ß3 (Cat. orb613674) subunit expression in the cytosolic (S2), extrasynaptic (non-PSD), and synaptic (PSD) fractions of untreated, bicuculline-pretreated (20μM, 1h), or netrin-1 treated (250ng/ml, 1h) rat hippocampal neuronal cultures.

Western blot of mouse brain lysates from wild type (WT) and α2-knockout (K/O) animals showing specific immunolabeling of the ~51 kDa α2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from α2-knockout animals.

Western blot of rat cortical neurons showing specific immunolabeling of the ~51 kDa α2-subunit of the GABAA-R (1:1000).

Quick Database Links

Gene Symbol

GABAA Receptor α2

UniProt

P23576