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Anti-FTO Antibody

Catalog Number: orb1474535

DispatchUsually dispatched within 5-10 working days
$ 170.00
Catalog Numberorb1474535
CategoryAntibodies
DescriptionRabbit monoclonal antibody to FTO
Species/HostRabbit
ClonalityMonoclonal
Tested applicationsIF, IH, WB
ReactivityHuman
ImmunogenA synthetic peptide of human FTO
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100)
Form/AppearanceLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
ConjugationUnconjugated
TargetFTO
Entrez79068
UniProt IDQ9C0B1
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
Alternative namesKIAA1752; Alpha-ketoglutarate-dependent dioxygenas
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-FTO Antibody

Western blot analysis of FTO expression in Hela (A), Jurkat (B) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 58 kD)

Anti-FTO Antibody

Immunohistochemical analysis of FTO staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.82). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-FTO Antibody

Immunofluorescent analysis of FTO staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

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    Human, Mouse, Rat

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    Polyclonal

    Unconjugated

    10 μg, 100 μg
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    Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep

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    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg