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Anti-Frizzled 1/2/7 Antibody
Catalog Number: orb256544
| Catalog Number | orb256544 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Frizzled 1/2/7 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Frizzled 1/2/7. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:500-1000 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | FZD1; FZD2; FZD7 |
| Entrez | 8324, 14369 |
| UniProt ID | O75084, Q9UP38, Q14332, Q61090 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | anti Frizzled-7 antibody, anti Fz-7 antibody, anti Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Frizzled 1/2/7 expression in Hela (A), mouse heart (B), rat heart (C), rat kidney (D) whole cell lysates. (Predicted band size: 71; 63 kD; Observed band size: 64; 71 kD)
Immunofluorescent analysis of Frizzled 1/2/7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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