Anti-FOLR1 [Farletuzumab (MORAb-003; M3)]

• Catalog Number: orb1906392
• Category: Antibodies
• Description: Anti-FOLR1 [Farletuzumab (MORAb-003; M3)]
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: Farletuzumab (MORAb-003; M3)
• Tested applications: ELISA, FA, Functional Studies, IHC, In vivo, NeA
• Reactivity: Human, Primate
• Isotype: IgG
• Immunogen: The original parental mouse antibody was generated by immunizing (BALB/c X C57BL/6) F1 mice by intraperitoneal injection containing cultured LU-75(c) choriocarcinoma cells to produce hybridomas that generated the antibody LK26. Later on the Farletuzumab was generated by CDR grafting technique by taking CDRs of mouse parental clone LK26 and grafting them on human framework regions.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: FOLR1
• UniProt ID: P15328
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: FRA; Frα; Fr-α; FBP; LK26; Folate receptor 1; Fola
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of HeLa cells with anti-FOLR1 antibody Farletuzumab (MORAb-003; M3). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with Farletuzumab (MORAb-003; M3) (orb1906392) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906392, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-FOLR1 antibody Farletuzumab (MORAb-003; M3). HeLa cells lysate (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with Farletuzumab (MORAb-003; M3) (orb1906392) at a concentration of 2 µg/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.