Anti-FOLR1 [Farletuzumab (MORAb-003; M3)]

SKU: orb1906392

Description

Anti-FOLR1 [Farletuzumab (MORAb-003; M3)]

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FA, Functional Studies, IHC, In vivo, NeA
• Reactivity: Human, Primate

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: Farletuzumab (MORAb-003; M3)
• Immunogen: The original parental mouse antibody was generated by immunizing (BALB/c X C57BL/6) F1 mice by intraperitoneal injection containing cultured LU-75(c) choriocarcinoma cells to produce hybridomas that generated the antibody LK26. Later on the Farletuzumab was generated by CDR grafting technique by taking CDRs of mouse parental clone LK26 and grafting them on human framework regions.
• Target: FOLR1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FRA; Frα; Fr-α; FBP; LK26; Folate receptor 1; Folate receptor alpha; FR-alpha; Adult folate-binding protein; Ovarian tumor-associated antigen MOv18; KB cells FBP; Folate receptor adult; MOv18

Immunofluorescence staining of HeLa cells with anti-FOLR1 antibody Farletuzumab (MORAb-003; M3). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with Farletuzumab (MORAb-003; M3) (orb1906392) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906392, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-FOLR1 antibody Farletuzumab (MORAb-003; M3). HeLa cells lysate (35 µg protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with Farletuzumab (MORAb-003; M3) (orb1906392) at a concentration of 2 µg/ml before detection using an appropriate secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

Quick Database Links

Gene Symbol

FOLR1

UniProt

P15328