Focal Adhesion Kinase (Phospho-Y861) Rabbit Polyclonal Antibody

SKU: orb214478

Description

The Focal Adhesion Kinase (Phospho-Y861) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y861 of human Focal Adhesion Kinase protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1-500-1-1000, IP: 1-10-1-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y861 of human Focal Adhesion Kinase protein. The exact sequence is proprietary.
• Target: PTK2
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FAK; FAK1; Focal adhesion kinase 1; FADK 1; Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK

Western blot analysis of Focal Adhesion Kinase (Phospho-Y861) expression in mouse kidney (A), rat kidney (B) whole cell lysates. (Predicted band size: 119; 99; 63; 48; 120; 39; 114 kD; Observed band size: 100 kD)

Immunohistochemical analysis of Focal Adhesion Kinase (Phospho-Y861) staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

PTK2

UniProt

Q05397

NCBI Gene ID

5747