FNDC5 Rabbit Polyclonal Antibody

SKU: orb1879467

Description

The FNDC5 Antibody is suitable for FC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human FNDC5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human FNDC5. The exact sequence is proprietary.
• Target: FNDC5
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FRCP2; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2

Western blot analysis of FNDC5 expression in K562 (A) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 28 kD)

Flow cytometric analysis of K562 cells using Anti-FNDC5 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Quick Database Links

Gene Symbol

FNDC5

UniProt

Q8NAU1

NCBI Gene ID

252995