Anti-Fibronectin [G4]

SKU: orb411581

Description

Human monoclonal antibody to ED-B domain of fibronectin

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Molecular Biology, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC-Fr, WB
• Reactivity: Human

Key Properties

• Host: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: G4
• Immunogen: 2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells.
• Target: Fibronectin
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EDB(+) FN; ED-B domain of fibronectin;Extra domain-B containing fibronectin

Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody G4. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of G4 (orb411582) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb411582, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Fibronectin

UniProt

P02751

Pini, A. et al. Design and use of a phage display library. Human antibodies with subnanomolar affinity against a marker of angiogenesis eluted from a two-dimensional gel J Biol Chem, 273, 21769-21776 (1998)

PubMed: 9705314