Anti-Fibrin beta-chain [8E5]

• Catalog Number: orb613934
• Category: Antibodies
• Description: Mouse monoclonal antibody to Fibrin betachain
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8E5
• Tested applications: ELISA
• Reactivity: Human
• Isotype: IgG1
• Immunogen: 8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic β-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Fibrin beta-chain
• UniProt ID: P02675
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Factor Ia; factor-1a; fibrin; fibrin β chain; fibr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-Fibrin beta-chain antibody 8E5 HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of 8E5 (orb613935, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.