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Anti-EYA4 Antibody

Catalog Number: orb1879403

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1879403
CategoryAntibodies
DescriptionRabbit polyclonal antibody to EYA4
ClonalityPolyclonal
Tested applicationsFC, IH, WB
ReactivityHuman
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human EYA4. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/200), FC (1/10 - 1/50)
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
Entrez2070
UniProt IDO95677
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesEyes absent homolog 4
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-EYA4 Antibody

Western blot analysis of EYA4 expression in A2058 (A) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 52 kD)

Anti-EYA4 Antibody

Immunohistochemical analysis of EYA4 staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-EYA4 Antibody

Flow cytometric analysis of HL60 cells using Anti-EYA4 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

  • Goat anti-EYA4 Antibody [orb19229]

    ELISA,  IHC

    Human, Mouse

    Goat

    Polyclonal

    Unconjugated

    100 μg
  • Anti-EYA4 Antibody [orb654424]

    ELISA,  FC,  IHC,  WB

    Human, Monkey, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • EYA4 Antibody [orb417723]

    ELISA,  IF,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 50 μg
  • Anti-EYA1 Antibody [orb234835]

    IH,  WB

    Human, Mouse, Primate, Rat, Zebrafish

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Rabbit anti-EYA4 Antibody [orb1526287]

    IHC,  IP,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    2 μg