Anti-Estrogen Receptor alpha Antibody

• Catalog Number: orb1474541
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Estrogen Receptor alpha
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: ChIP, IF, IH, IP, WB
• Reactivity: Human
• Immunogen: A synthetic peptide of human Estrogen Receptor alpha
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: ESR1
• Entrez: 2099
• UniProt ID: P03372
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: ESR; NR3A1; Estrogen receptor; ER; ER-alpha; Estra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Estrogen Receptor alpha expression in K562 (A), Hela (B) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 66 kD)

Immunohistochemical analysis of Estrogen Receptor alpha staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.11). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Estrogen Receptor alpha staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.