ERK7 Mouse Monoclonal Antibody

SKU: orb1473722

Description

The ERK7 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human ERK7. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant fusion protein of human ERK7. The exact sequence is proprietary.
• Target: MAPK15
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8

Western blot analysis of ERK7 expression in MCF7 (A) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 59 kD)

Immunofluorescent analysis of ERK7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

MAPK15

UniProt

Q8TD08

NCBI Gene ID

225689