ERK2 Rabbit Monoclonal Antibody

SKU: orb546992

Description

Anti-ERK2 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:40 FC 1:40
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: II-13
• Immunogen: A synthesized peptide derived from human ERK2 Act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of ERK2 requires its phosphorylation by upstream kinases. ERK2 is located in the cytoplasm of resting cells and translocates into the nucleus upon extracellular stimuli by active transport of a dimer. ERK2 is essential for placental development and ERK2 in the trophoblast compartment may be indispensable for the vascularization of the labyrinth.
• Target: Mitogen-activated protein kinase 1
• Molecular Weight: 131 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF

WB analysis of MAPK1 expression in (1) A431 Cell; (2) HeLa Cell using anti-MAPK1 antibody.

IHC analysis of MAPK1 using anti-MAPK1 antibody.MAPK1 was detected in paraffin-embedded section.

Quick Database Links

Gene Symbol

Mitogen-activated protein kinase 1

UniProt

P28482