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Anti-ERCC2 Antibody
Catalog Number: orb412366
| Catalog Number | orb412366 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to ERCC2 |
| Target | ERCC2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Rat |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Immunogen | Recombinant full length protein of human ERCC2 |
| UniProt ID | P18074 |
| Tested applications | IF, WB |
| Dilution range | WB: 1:500-2000, IF/ICC: 1:50-200 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti-XPD antibody, anti-XPDC antibody, anti-TFIIH Read more... |
| Note | For research use only |
| Entrez | 2068 |
Western blot analysis of ERCC2 expression in THP1 (A), HepG2 (B) whole cell lysates. (Predicted band size: 46; 86 kD; Observed band size: 87 kD)
Immunofluorescent analysis of ERCC2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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