Anti-ERBB2 [SAIC-02A-7]

• Catalog Number: orb1090000
• Category: Antibodies
• Description: Rabbit monoclonal antibody to ERBB2
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: SAIC-02A-7
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide "GLQSLPTHDPSPLQR" derived from the receptor tyrosine kinase erbB2 conjugated to KLH.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: ERBB2
• UniProt ID: P04626
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: HER2; MLN19; NEU; NGL; Receptor tyrosine-protein k
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-ERBB2 antibody SAIC-02A-7. MCF7 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG1 version of SAIC-02A-7 (orb1090000, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells using anti-ERBB2 antibody SAIC-02A-7. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of SAIC-02A-7 (orb1090000) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1090000, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.