EphrinB (Tyr317) Antibody

SKU: orb319512

Description

Our Anti-EphrinB (Tyr317) rabbit polyclonal phosphospecific primary antibody from Biorbyt is produced in-house. It detects mouse and rat EphrinB (Tyr317) and is antigen affinity purified from pooled serum.

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB: 0.736111111
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Gallus, Human, Xenopus, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Tyr317 of chicken EphrinB, conjugated to keyhole limpet hemocyanin (KLH). Note: Chicken Tyr317 is the homolog of rat and mouse Tyr328, human Tyr329 and Xenopus Tyr310.
• Target: EphrinB (Tyr317)
• Molecular Weight: 46
• Purification: Antigen Affinity Purified from Pooled Serum
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml rAlbumin and 50% glycerol.
• Concentration: Lot Dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CEK5 ligand antibody, EFNB1 antibody, ELK L antibody, ELK ligand antibody, ELKL antibody, EPH related receptor tyrosine kinase ligand 2 antibody, Ephrin B1 antibody, Ephrin B2 antibody, LERK2 antibody

Western blot of rat testes lysate showing specific immunolabeling of the ~46 kDa EphrinB phosphorylated at Tyr317 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Quick Database Links

Gene Symbol

EphrinB (Tyr317)

UniProt

P28693