Anti-EPHA2 (Phospho-Y588/596) Antibody

• Catalog Number: orb393275
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EPHA2 (Phospho-Y588/596)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y588/596 of human EPHA2 protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500:1000, IF/ICC: 1:100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EPHA2; EPHA3; EPHA4
• Entrez: 2043, 13836, 2042, 13838, 29210, 1969
• UniProt ID: Q03145, Q03137, O08680, P54764, P29320, P29317, P29319
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: EPHA2; ECK; Ephrin type-A receptor 2; Epithelial c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EPHA2 (Phospho-Y588/596) expression in A431 (A), mouse brain (B) whole cell lysates. (Predicted band size: 108; 110; 109 kD; Observed band size: 130 kD)

Immunofluorescent analysis of EPHA2 (Phospho-Y588/596) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).