eNOS Antibody

• Catalog Number: orb323138
• Category: Antibodies
• Description: Mouse monoclonal antibody to eNOS
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: Affinity chromatography
• Immunogen: Recombinant protein corresponding to human eNOS.
• UniProt ID: Q62600, P70313, P29474
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:200-1:500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Nitric oxide synthase endothelial; Constitutive NO
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 18127, 4846, 24600
• Expiration Date: 12 months from date of receipt.

Western blot analysis of eNOS expression in rat heart (A) whole cell lysates. (Predicted band size: 133; 69 kD; Observed band size: 133 kD)

Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.