eNOS Mouse Monoclonal Antibody

SKU: orb323138

Description

The eNOS Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human eNOS. Purification: Affinity chromatography

Research Area

Epigenetics

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500-1:2000, IHC-P: 1:200-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant protein corresponding to human eNOS.
• Target: NOS3
• Purification: Affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nitric oxide synthase endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

Western blot analysis of eNOS expression in rat heart (A) whole cell lysates. (Predicted band size: 133; 69 kD; Observed band size: 133 kD)

Immunohistochemical analysis of eNOS staining in mouse colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

NOS3

UniProt

P70313, P29474, Q62600

NCBI Gene ID

18127, 24600, 4846