Anti-EMR3 Antibody

• Catalog Number: orb515573
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EMR3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EMR3. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EMR3
• Entrez: 84658
• UniProt ID: Q9BY15
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: EGF-like module-containing mucin-like hormone rece
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EMR3 expression in HEK293T (A), H1975 (B), mouse liver (C), mouse kidney (D), rat liver (E), rat kidney (F) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 73 kD)

Immunofluorescent analysis of EMR3 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.