ELF5 Antibody

SKU: orb213890

Description

Rabbit polyclonal antibody to ELF5

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500, IP: 1-10-1-100
• Reactivity: Bovine, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ELF5. The exact sequence is proprietary.
• Target: ELF5
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

anti ESE2 antibody, anti ETS-related transcription factor Elf-5 antibody, anti E74-like factor 5 antibody, anti Epithelium-restricted ESE-1-related Ets factor antibody, anti Epithelium-specific Ets transcription factor 2 antibody, anti ESE-2 antibody

Western blot analysis of ELF5 expression in HeLa (A), HuvEc (B), Jurkat (C) whole cell lysates. (Predicted band size: 16; 18; 30; 31 kD; Observed band size: 31 kD)

Immunofluorescent analysis of ELF5 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

ELF5

UniProt

Q9UKW6, Q8VDK3

NCBI Gene ID

13711, 2001