Elafin/Skalp PI3 Mouse Monoclonal Antibody

SKU: orb421110

Description

Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7). Tested in ELISA, IHC applications. This antibody reacts with Human.

Research Area

Cell Biology, Immunology & Inflammation, Metabolism Research, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml ELISA (Cap), 1-5μg/ml
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: C7
• Immunogen: E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).
• Target: Elafin
• Molecular Weight: 25 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cementoin; Elafin; Elastase specific inhibitor; ESI; Peptidase inhibitor 3; PI 3; PI3; Protease inhibitor WAP3; SKALP; WAP3; WFDC14

IHC analysis of Elafin using anti-Elafin antibody. Elafin was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Elafin Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Sandwich ELISA - Recombinant human Elafin/Skalp protein standard curve. Use in combination with reagents from Human Elafin/Skalp ELISA Kit EZ-Set (DIY Antibody Pairs).

Quick Database Links

Gene Symbol

Elafin

UniProt

P19957