Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7)

• Catalog Number: orb421110
• Category: Antibodies
• Description: Anti-Elafin/Skalp PI3 Antibody (monoclonal, C7). Tested in ELISA, IHC applications. This antibody reacts with Human.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C7
• Tested applications: ELISA, IHC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 25 kDa
• UniProt ID: P19957
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Elafin; Elastase-specific inhibitor; ESI; Peptidas
• Note: For research use only
• Application notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml ELISA (Cap), 1-5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

IHC analysis of Elafin using anti-Elafin antibody. Elafin was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Elafin Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Sandwich ELISA - Recombinant human Elafin/Skalp protein standard curve. Use in combination with reagents from Human Elafin/Skalp ELISA Kit EZ-Set (DIY Antibody Pairs).