EIF4E Rabbit Monoclonal Antibody

SKU: orb547088

Description

The EIF4E Rabbit Monoclonal Antibody is an unconjugated antibody raised in rabbit. It is purified by affinity chromatography and suitable for use in FC, IHC, IP, and WB applications. This antibody targets the Eukaryotic translation initiation factor 4E in Human, Mouse, and Rat samples.

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Dilution Range: WB 1:1000-5000 IHC 1:50-200 IP 1:30 FC 1:40
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: BCHJKX7
• Immunogen: A synthesized peptide derived from human EIF4E eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
• Target: Eukaryotic translation initiation factor 4E
• Molecular Weight: 25 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Transcriptional repressor CTCF; 11-zinc finger protein; CCCTC-binding factor; CTCFL paralog; CTCF

Quick Database Links

Gene Symbol

Eukaryotic translation initiation factor 4E

UniProt

P06730