Anti-EIF4B Antibody

• Catalog Number: orb1474445
• Category: Antibodies
• Description: Rabbit monoclonal antibody to EIF4B
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: A synthetic peptide of human eIF4B
• Antibody Type: Primary Antibody
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: EIF4B
• Entrez: 1975
• UniProt ID: P23588
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: Eukaryotic translation initiation factor 4B; eIF-4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EIF4B expression in Hela (A), A549 (B), HL60 (C), U2OS (D) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 80 kD)

Immunohistochemical analysis of EIF4B staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.95). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.