EIF1 Antibody

• Catalog Number: orb256510
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EIF1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Gallus, Human, Monkey, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EIF1. The exact sequence is proprietary.
• UniProt ID: P41567, P48024
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SUI1; Eukaryotic translation initiation factor 1;
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 10209, 20918
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EIF1 expression in A549 (A), Raw264.7 (B), U87MG (C), THP1 (D), PC3 (E), rat lung (F), mouse spleen (G) whole cell lysates. (Predicted band size: 12 kD; Observed band size: 13 kD)

Immunofluorescent analysis of EIF1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).