Anti-EGFRvIII [806]

• Catalog Number: orb1906395
• Category: Antibodies
• Description: Anti-EGFRvIII [806]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 806
• Tested applications: FC, IHC, IP
• Reactivity: Human
• Isotype: IgG
• Immunogen: The original antibody was generated by immunization of mice with NR6 mouse fibroblasts expressing the truncated de2–7 EGFR.
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Target: EGFRvIII
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: de2–7 EGFR; EGF receptor; delta2–7 epidermal growt
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of A431 cells with anti-EGFRvIII antibody 806. Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 806 (orb1906395) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cell junction staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1906395, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-EGFRvIII antibody 806. A431 (A) (0.003 µg/ml), HeLa (B) (0.3 µg/ml), and MDA-MB-231 (C) (0.1 µg/ml) cells lysates (35 µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 806 (orb1906395) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.