Anti-EGFR [S3 12D]

• Catalog Number: orb613856
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Human EGFR.
• UniProt ID: P00533
• Tested applications: ELISA, ICC, IF, IHC, WB
• Clone Number: S3 12D
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Epidermal growth factor receptor; ERBB; ERBB1; HER
• Research Area: Cancer Biology, Immunology & Inflammation
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-EGFR S3 12D Mouse splenocytes were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of S3 12D (orb613857, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed NIH3T3 cells with anti-EGFR antibody S3 12D. Immunofluorescence analysis of unpermeabilisd paraformaldehyde fixed NIH3T3 cells on Shi-fix™ coverslips stained with the chimeric rabbit version of S3 12D (orb613857) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613857, DAPI, merged channels and an isotype control. The isotype control was stained with unimmunised rabbit IgG followed by Alexa Fluor® 488 secondary antibody.