EGFR Antibody (APC)

• Catalog Number: orb1821026
• Category: Antibodies
• Description: The oncoprotein EGFR (epidermal growth factor receptor), also known as HER1 / ErbB1, is a member of ErbB family of cell surface receptor tyrosine kinases. This 170 kDa transmembrane glycoprotein is often associated with cancerogenesis, although its activation state is controlled at various levels including trafficking and degradation steps. Binding of receptor-specific ligands to the EGFR ectodomain results in formation of homodimeric or heterodimeric kinase-active complexes into which HER2 / ErbB2 is recruited as a preferred partner. Subsequent signaling cascades such as RAS/MAPK and PI3K/AKT pathways lead to cell proliferation and survival responses.
• Target: EGFR
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Conjugation: APC
• Reactivity: Equine, Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Human epidermoid carcinoma line A431
• UniProt ID: P00533
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: EGFR1
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: EGF-R, Her1, ERBB1, PIG61, NISBD2
• Note: For research use only
• Entrez: 1956
• Expiration Date: 12 months from date of receipt.

Separation of A431 cells stained using anti-EGFR (EGFR1) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from A431 cells stained using mouse IgG2b isotype control (MPC-11) APC antibody (concentration in sample 5 μg/ml, same as EGFR APC antibody concentration, black-dashed) in flow cytometry analysis (surface staining).