Anti-EGFR [528]

• Catalog Number: orb348873
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Partially purified EGFR from A431 cells.
• UniProt ID: P00533
• Tested applications: FC, IF, IHC-P, IP, WB
• Clone Number: 528
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB; ERBB1; HER1; PIG61; mENA; avian erythroblast
• Research Area: Cancer Biology, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-EGFR antibody 528 HeLa (A) and A431 (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 528 (orb348897, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analysed on a FACSCanto flow-cytometer.

Immunofluoresence staining of fixed HeLa cells with anti-EGFR antibody 528. Panels show from left-right, top-bottom orb348897, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.