Anti-EGFR [2F8 (Zalutumumab; HuMax-EGFR)]

• Catalog Number: orb1152477
• Category: Antibodies
• Description: Human monoclonal antibody to EGFR
• Target: EGFR
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The original monoclonal antibody was generated by immunizing HuMAb mice with alternating A431 cells and purified EGFR administration.
• UniProt ID: P00533
• Tested applications: ELISA, FC, WB
• Clone Number: 2F8 (Zalutumumab; HuMax-EGFR)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ERBB1; ERBB; Erb-B2; HER1; Epidermal growth factor
• Research Area: Cancer Biology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-EGFR antibody 2F8 (Zalutumumab; HuMax-EGFR). Paraformaldehyde fixed A431 cells, permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 2F8 (Zalutumumab; HuMax-EGFR) (orb1152476, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of A431 cells with anti-EGFR 2F8 (Zalutumumab; HuMax-EGFR). Immunofluorescence analysis of paraformaldehyde fixed A431 cells stained with the chimeric rabbit IgG version of 2F8 (Zalutumumab; HuMax-EGFR) (orb1152476)(1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cellular junction staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152476, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.