EEF2 Mouse Monoclonal Antibody

SKU: orb1474361

Description

The EEF2 Antibody is suitable for ChIP, IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant human eEF2 protein fragments expressed in E.coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: ChIP, IF, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), ChIP (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human eEF2 protein fragments expressed in E.coli.
• Target: EEF2
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EF2; Elongation factor 2; EF-2

Western blot analysis of EEF2 expression in HL60 (A), Jurkat (B), SHSY5Y (C), U20S (D), Hela (E) whole cell lysates. (Predicted band size: 95 kD; Observed band size: 95 kD)

Immunofluorescent analysis of EEF2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

EEF2

UniProt

P13639

NCBI Gene ID

1938