Anti-EDAR [EDAR12]

• Catalog Number: orb613920
• Category: Antibodies
• Description: Mouse monoclonal antibody to EDAR
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: EDAR12
• Tested applications: ELISA, WB
• Reactivity: Canine, Gallus, Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: EDAR
• UniProt ID: Q9R187
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: ectodysplasin A receptor; Tumor necrosis factor re
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed A549 cells with anti-EDAR antibody EDAR12. Immunofluorescence analysis of paraformaldehyde fixed A549 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of EDAR12 (orb613920) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing weak membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613920, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.