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Item 1 of 2
| Catalog Number | orb234827 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to EB2 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, WB |
| Reactivity | Human, Mouse, Primate, Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EB2. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:500-1000 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | MAPRE2 |
| Entrez | 212307, 10982, 679221 |
| UniProt ID | Q8R001, Q3B8Q0, Q15555 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | anti RP1 antibody, anti Microtubule-associated pro Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
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Stefano Pezzini 1, Aurora Mustaccia 2, Pierre Aboa 1, Giorgia Faustini 1, Alessio Branchini 3, Mirko Pinotti 3, Angelisa Frasca 1, Joseph J Porter 4, John D Lueck 4, Nicoletta Landsberger Engineered tRNAs efficiently suppress CDKL5 premature termination codons Engineered tRNAs efficiently suppress CDKL5 premature termination codons, Sci Rep, (2024)
Applications
WB
Reactivity
Human
Western blot analysis of EB2 expression in mouse heart (A), rat heart (B) whole cell lysates. (Predicted band size: 37 kD; Observed band size: 50 kD)
Immunofluorescent analysis of EB2 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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