Anti-E Cadherin [4A2]

SKU: orb2645612

This product can be custom made

Description

Research Area

Cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	FC, IF, IHC, IP, WB
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	4A2
Immunogen	The original mouse IgG1 version of this antibody was raised by immunizing mice with the recombinant maltose binding protein fused to full length human E-Cadherin.
Target	E Cadherin
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Cadherin-1; CD324; Cadherin E; E-Cadherin; epithelial cadherin; CAM 120/80; Uvomorulin

Quality Guarantee

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Flow-cytometry using the anti-E Cadherin antibody 4A2. MCF7 cells were stained with an isotype control (orb256390, black line) or the rabbit-chimeric version of 4A2 (orb2645612, blue line) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488® conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells with anti-E Cadherin 4A2. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of 4A2 (orb2645612) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645612, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-E Cadherin antibody 4A2. MCF7 cells (0.0001 µg/ml) lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 4A2 (orb2645612) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.