Anti-E Cadherin [19A11]

• Catalog Number: orb1821552
• Category: Antibodies
• Description: Anti-E Cadherin [19A11]
• Target: E Cadherin
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Canine, Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: The original antibody was generated by immunizing mice with purified ECad1-5 protein.
• UniProt ID: P12830
• Tested applications: ELISA, FC, Functional Studies, WB
• Clone Number: 19A11
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD324; Cadherin-1; Cadherin E; E-Cadherin; epithel
• Note: For research use only

Flow cytometry using anti-E Cadherin antibody 19A11. MCF7 cells were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 19A11 (orb1821551, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of MCF7 cells with anti-E Cadherin antibody 19A11. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 19A11 (orb1821551) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821551, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-E Cadherin antibody 19A11. MCF7 (A) (0.03 µg/ml) and Caco-2 (B) (0.01 µg/ml) cells lysate (35 µg protein in RIPA buffer) were resolved on an SDS-PAGE gel, and blots were probed with the chimeric rabbit version of 19A11 (orb1821551) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and protein was detected by chemiluminescence.