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Anti-DOS Antibody
Catalog Number: orb1879413
| Catalog Number | orb1879413 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to DOS |
| Clonality | Polyclonal |
| Tested applications | FC, WB |
| Reactivity | Human |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the Central region of human DOS. The exact sequence is proprietary. |
| Antibody Type | Primary Antibody |
| Dilution range | WB (1/500 - 1/1000), FC (1/10 - 1/50) |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Entrez | 255057 |
| UniProt ID | Q8N350 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | C19orf26; Protein Dos Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of DOS expression in MDAMB435 (A), Jurkat (B) whole cell lysates. (Predicted band size: 73 kD; Observed band size: 63 kD)
Flow cytometric analysis of MDAMB435 cells using Anti-DOS Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
