Anti-DNA Polymerase zeta Antibody

• Catalog Number: orb315685
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DNA Polymerase zeta also known as hREV3 and REV3-like whose expression can be detected in the whole body ubiquitously. It is found in the nucleus. It can perform various functions like DNA-dependent DNA replication. This protein lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function but has a catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translation DNA synthesis (TLS).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Polymerase zeta. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: REV3L
• Entrez: 5980, 19714
• UniProt ID: Q61493, O60673
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-POLZ antibody, anti-REV3 antibody, anti-DNA p
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DNA Polymerase zeta expression in rat lung (A), mouse kidney (B), H1792 (C) whole cell lysates. (Predicted band size: 352 kD; Observed band size: 350 kD)

Immunohistochemical analysis of DNA Polymerase zeta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of DNA Polymerase zeta staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.