DNA-PKcs Mouse Monoclonal Antibody

SKU: orb1474214

Description

The DNA-PKcs Antibody is suitable for IF, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant human DNA-PKcs protein fragments expressed in E.coli .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human DNA-PKcs protein fragments expressed in E.coli
• Target: PRKDC
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460

Western blot analysis of DNA-PKcs expression in Hela (A), K562 (B), HL60 (C), Molt4 (D) whole cell lysates. (Predicted band size: 469 kD; Observed band size: 450 kD)

Immunofluorescent analysis of DNA-PKcs staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

PRKDC

UniProt

P78527

NCBI Gene ID

5591