DNA Ligase 4 Rabbit Polyclonal Antibody

SKU: orb338995

Description

The DNA Ligase 4 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DNA Ligase 4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cancer, DNA Damage

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DNA Ligase 4. The exact sequence is proprietary.
• Target: LIG4
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4

Western blot analysis of DNA Ligase 4 expression in KB (A), MCF7 (B) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 127 kD)

Immunohistochemical analysis of DNA Ligase 4 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

LIG4

UniProt

Q8BTF7, P49917

NCBI Gene ID

319583, 3981