Anti-DNA Ligase 4 Antibody

• Catalog Number: orb338995
• Category: Antibodies
• Description: Rabbit polyclonal antibody to LIG4
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DNA Ligase 4. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: LIG4
• Entrez: 3981
• UniProt ID: P49917
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti DNA ligase 4 antibody, anti DNA ligase IV ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DNA Ligase 4 expression in KB (A), MCF7 (B) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 127 kD)

Immunohistochemical analysis of DNA Ligase 4 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.